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Clinical significance of maternal serum markers to detect infectious pathogens

Author: WangAiXiang,LiuXiaoFeng From: www.yourpaper.net Posted: 2010-06-24 11:57:04 Read:
[Abstract] purpose to explore the maternal (pre-) clinical significance of serum markers to detect infectious pathogens. The method using enzyme-linked immunosorbent assay (ELISA) detected 5639 cases of maternal hepatitis B surface antigen (HBsAg), hepatitis C antibody (anti-HCV), HIV 1/2 antibody (anti-HIV1 / 2), syphilis serology ( TRUST) to detect syphilis antibodies. 5639 cases of maternal HBsAg positive patients, 270 patients (4.7%), 5 cases of hepatitis C antibody positive patients (0.08%), patients with positive screening HIV antibody 1/2 in 3 cases (0.05%), disease control by the Qingdao Municipal Center AIDS confirm that the laboratory has confirmed positive patients in 3 cases, 3 cases of syphilis (TRUST) positive patients (0.05%). Conclusion maternal prenatal serum markers in early detection of positive patients, prevention and control of vertical transmission to avoid occupational infection of medical workers, is of great significance.
the [Key words] maternal; infectious pathogens serum markers; hepatitis B surface antigen; hepatitis C antibody; HIV 1/2 antibody; syphilis
With the improvement of people's living standards, health awareness and legal awareness has gradually increased, in order to prenatal and postnatal care, reduce medical disputes, our hospital on maternal prenatal serum markers testing, including hepatitis B surface antigen (HBsAg), hepatitis C antibody (anti-HCV), HIV-1/2 antibody (anti-HIV1 / 2), syphilis antibodies.
Materials and Methods
1.1 Object Detection 5639 cases of clinical serum samples from our hospital outpatient maternal.
1.2 Reagents and Instruments
1.2.1 Reagents HBsAg, anti-HCV, anti-HIV1 / 2 (Xiamen Intco, New Technology Co., Ltd.). Syphilis (Shanghai Rongsheng Biotechnology Co., Ltd.). TPPA (Japan Fu Guthrie will Europe shares will).
1.2.2 Instrument microplate reader (the Merieux CliniBi0-128C), enzyme-linked immunosorbent biological accelerometer (the Fosun NY / MMJ type), washer (Abbott), the oscillator.
1.3 Detection of HBsAg, anti-HCV, anti-HIV1 / 2 enzyme-linked immunosorbent assay (ELISA), all in strict accordance with the instructions, daily with internal quality control (all participated in the Ministry of Health and the provincial chamber QC score of 100 points). Qingdao Municipal Centers for Disease Control AIDS anti-HIV positive screening according to prescribed procedures to send confirmation laboratory for confirmation. Syphilis serum test assay, toluidine red heating the positive titer experiments and TPPA a confirmatory test.
2 test results
HBsAg-positive patients, 270 cases (4.7%), 5 cases of hepatitis C antibody positive patients (0.08%), HIV antibody positive patients, 3 patients (0.05%) is recognized by the Qingdao Municipal Center for Disease Control, three cases of syphilis antibody positive patients (0.05%) Trust positive titer experiment and TPPA confirmatory test, including 2 cases titer of 1:32 positive, TPPA positive; 1 cases titer of the original times positive, TPPA were negative. Table 1. Table 15 639 cases of pregnant women to detect the infectious markers positive rate
The 5639 cases serum infectious markers positive rate was 5.3%, non-overlapping infections. The highest infection rate was 4.7% of HBsAg in the detection of indicators, maternal carriers of hepatitis B virus in the region is the main source of infection. In order to reduce and block vertical transmission, the Court of perinatal maternal adequate preparations. (1) try to shorten the production process, to reduce the risk of maternal blood seeped into the fetus, to protect infants against the pollution of the maternal blood and secretions, pay special attention to avoid the damage of neonatal skin and mucous membranes [1]. (2) newborn within 6h hepatitis B immunoglobulin white of 200IU joint hepatitis B DNA vaccine (5 microg), two weeks after the injection of hepatitis B immunoglobulin white (200IU), 1,6 months after the injection of a hepatitis B gene vaccine (5 g). (3) detecting maternal content of HBV-DNA in 104 the emboss / ml above prohibited breastfeeding. According to the study, the anti-HCV among the healthy, positive rate of 0.7% to 1.3% [2], the detection of the positive rate was 0.08%, although the infection is low, but a lot of foreign data confirm the presence of HCV vertical transmission [3,4], to remain vigilant to prevent undetected. By the mainland to further open up and an increase in staff and external communication, human immunodeficiency disease (AIDS) has shown an increase trend, the detection of anti-HIV screening positive patients and in 3 cases, Qingdao City, the Centers for Disease Control AIDS has been recognized laboratory to confirm three pregnant women are foreign workers, the loss of the test results, can not be traced.
Treponema pallidum can be active placenta of pregnant women vertical transmission to the baby. Prenatal syphilis screening has become one of the many countries syphilis and congenital syphilis control measures [5]. The detection titer experiments and TPPA confirmatory test, with the the TRUST Act primary screening syphilis antibody positive patients three cases and two cases of pregnant women TRUST titer of 1:32 positive, TPPA positive, its birth and 6 months check TPPA is positive, the need to follow-up investigation. Pregnant women TRUST titer of times positive, TPPA were negative, a month after the review TRUST and TPPA were negative, and 1 case of false positive.
A lot of information at home and abroad that HBsAg, anti-HCV, syphilis, anti-HIV vertical transmission through the placenta, thus enhancing the perinatal care, positive patients with early detection, prevention and control of mother-to-child vertical transmission, to prevent cross infection, avoid medical workers caused by contact with the spread of great significance for their surgery, clinics and other invasive procedures.
1 Cai Xia, Zheng Meiyu, Wang Sai Kong. Impact of hepatitis C on maternal and fetal Chinese Journal of Gynecology and Obstetrics, 1996,13 (5): 337.

Peng Wenwei. Infectious Diseases, 5th Edition, Beijing: People's Health Publishing House ,2001:18-19.

3 Wene AJ, Thaler MM, Crawford KA unique predominant hepatt found in an infang bern to a morther with multiple vatlants Jvirol, 1993,67 (7): 4365.

4 Wwistal R.widel A mansson AS mothor-to-nfant transmission of hepants comments Ana Intern med, 1992,117 (11): 887.

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