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About Ganoderma spore oil righting the attenuation research papers

Author: WeiZhi From: www.yourpaper.net Posted: 2009-06-29 21:23:15 Read:
[keywords] Ganoderma spore oil;, immune,;, cyclophosphamide
Abstract: Objective To observe the Ganoderma spore oil righting attenuated role. Normal and H22 tumor by detecting the number of peripheral WBC number of nucleated cells, bone marrow, spleen index and thymus index to observe the oral Ganoderma spore oil (2.5,1.25,0.625 g / kg) for cyclophosphamide (Cy) chemotherapy and radiotherapy 60Co attenuated role; mice carbon clearance test, the serum hemolysin determination of experimental detection of drugs on the immune function in H22 tumor-bearing mice. Ganoderma spore oil in the high dose group can significantly confrontation, 60Co irradiation caused by high-dose Cy normal and tumor-bearing mice peripheral WBC number of nucleated cells in bone marrow, spleen and thymus index decreased (P <0.01, P <0.05 ). Ganoderma spore oil could significantly increase the dose of the phagocytic coefficient of tumor-bearing mice in the high dose group can significantly improve the tumor-bearing mice serum hemolysin (P <0.01, P <0.05). Conclusion Ganoderma spores oil can reduce Cy and 60Co-induced bone marrow suppression toxic, and can be significantly improved the mouse cellular and humoral immune function.
Keywords: Ganoderma lucidum spore oil; immune; cyclophosphamide

Ganoderma is a the motherland medicine treasure trove of precious medicines, our earliest pharmacy monograph "Shen Nong's Herbal Classic" classified as "top grade". Based on of Ganoderma exact clinical role in recent years, its chemical composition and the role of the mechanism of the large number of studies, the germ cells - the spores of Ganoderma lucidum medicinal value study also intensified. Previous studies have shown that the the Ganoderma spore has significant anti-cancer, anti-tumor effect, its efficacy is 60 to 70 times that of the fruiting bodies of Ganoderma lucidum. Ganoderma spore oil used in this experiment is the use of supercritical CO2 extraction technology enrichment derived from glossy ganoderma spores extracted and concentrated, the content of various active ingredients (such as triterpenoids) than those of traditional extraction methods significantly improve. The experimental study of tumor-bearing mice immunomodulatory and conventional radiotherapy and chemotherapy the attenuated role, in order to provide experimental evidence for clinical anti-tumor helper application Ganoderma spore oil.
1 Materials
1.1 Drug Ganoderma spore oil ganoderma spore oil by Guangzhou Modern Chinese Medicine Research and Development Co., Ltd., batch number: 050,601, before use of soybean oil diluted with concentration. The versicolor the spore within glycopeptide capsule, Nanjing Laoshan Pharmaceutical Co., Ltd., batch number: 050104, specifications: 0.5 g 21 capsules before use with a saline solution into the concentrations used. Injection with cyclophosphamide, Shanghai Hualian Pharmaceutical Co., Ltd., Specification: 200 mg / bottle, lot number: 020804.
1.2 tumor strain and animal H22 mouse hepatoma cells, provided by the Shanghai Institute of Materia Medica, Chinese Academy of Sciences. ICR mice, male and female, 18 to 22 g animal room provided by the China Pharmaceutical University, Certificate of Conformity: the Su kinoplasm SCXK (Su) 2002-0011.
2 methods
2.1 Ganoderma spore oil normal H22 tumor-bearing mice with chemotherapy (Cy) Attenuation take 60 ICR mice transplanted tumor research [1] inoculated H22 solid tumors, 24 h after randomization experimental design: control group, soybean oil control group (20 ml / kg), Ganoderma lucidum spore oil is high in the low dose group (2.5,1.25,0.625 g / kg) ig administered versicolor intracellular glycopeptide. capsule group (1 g / kg) ig administration. Another 60 ICR mice not vaccinated tumor-bearing, the grouping method of administration above. Groups administered 1 times / d, a total of seven times, each group in the administration 4,5 days (except blank group), the continuous 2 d ip Cy (100 mg / kg), after stopping for 24 h, group orbital venous blood, microscopic examination of peripheral blood WBC count, and the removal of the side of the intact femur, thymus, spleen and bone marrow were measured number of nucleated cells, known as the thymus, spleen weight, calculate the thymus, spleen coefficient, and statistical processing (t test).
2.2 Ganoderma spore oil in normal mice, the H22 tumor-bearing mice 60Co radiation attenuated role taken ICR mice of 60, transplanted tumor research [1], inoculated H22 solid tumors, 24 h after addition blank control group of 10 animals, the remaining 50 mice 60Co radiation dose of 500 rad; 60Co irradiation animals were randomly divided into five groups, the experimental design: blank control group, soybean oil control group (20 ml / kg), Ganoderma spore The oil is high in the low-dose group (2.5,1.25,0.625 g / kg) ig administration versicolor intracellular glycopeptide capsule group (1 g / kg) ig administration. Another 60 ICR mice not vaccinated tumor-bearing, in addition to the blank control group of 10 animals, the remaining 50 mice a 60Co radiation dose of 500 rad; animals were randomly divided into five groups of 60 Co irradiation, grouping, and to drug method ibid. Each group were administered 1 / d, a total of seven times, after stopping for 24 hours, each group orbital venous blood, microscopic examination of peripheral blood WBC count, while the removal of the side of the intact femur, thymus, spleen, were measured in the bone marrow number of nucleated cells, known as the thymus, spleen weight, calculate the thymus, spleen index, and for statistical analysis (t test).
2.3 Ganoderma spore oil the H22 tumor reticuloendothelial system phagocytosis of to take 50 ICR mice transplanted tumor research [1] inoculated H22 solid tumor. The next day were randomly divided into five groups, experimental design: soybean oil control group (20 ml / kg), Ganoderma lucidum spore oil high, medium, low dose group (2.5,1.25,0.625 g / kg) ig administration versicolor intracellular sugar peptide capsule group (1 g / kg) ig administration. Administered a total of 11 times. After the last administration for 24 h, the intravenous injection of India ink injected 1 min and 5 min from mouse orbital venous plexus blood immediately blown into Na2CO3 solution at 680 nm than the color, is calculated by the following formula clearance index K and phagocytic coefficient values. The resulting data for statistical analysis (t test)
Clearance index K = (logOD1-logOD5) / (t5-t1)
Phagocytosis coefficient = (weight / liver and spleen aggregate weight) x (K) 1/3

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2.4 Ganoderma spore oil H22 tumor humoral immune function take 50 ICR mice. Transplanted tumor research [1] inoculated H22 solid tumor. The next day were randomly divided into five groups, experimental design: soybean oil control group (20 ml / kg) ig administration, Ganoderma lucidum spore oil high, medium, low dose group (2.5,1.25,0.625 g / kg) ig administration versicolor intracellular glycopeptide capsule group (1 g / kg) ig administration. Total administration 11 days to seven days, each group mice by intraperitoneal injection of sheep red blood cells 0.2 ml / (20%) of the initial immunization, 1 h after the last administration, the blood, the serum was separated, measured and calculated hemolysin HC50, t-test between groups, the difference was significant.
3 Results
3.1 Ganoderma spore oil in normal mice, the H22 tumor-bearing mice chemotherapy (Cy) attenuation results in Table 2 shows, compared with the control group, Cy can significantly inhibit normal mice and tumor-bearing mouse peripheral blood WBC, bone marrow nucleated cells in the thymus and spleen coefficient (P <0.01). High dose group and versicolor intracellular glycopeptide group compared with the the Cy model of group, Ganoderma lucidum spore oil can fight of H22 tumor and normal mice spleen index, thymus, WBC count and bone marrow nucleated cells decreased (P <0.05 or P <0.01).
Table 1 Ganoderma spore oil Attenuation of normal mice chemotherapy (slightly)
Compared with the control group, P <0.05, P <0.01; compared with soybean oil Cy group, * P <0.05, ** P <0.01, n = 10
Ganoderma spore oil the H22 tumor chemotherapy Attenuation (omitted)
Compared with the control group, P <0.05, P <0.01; compared with soybean oil Cy group, * P <0.05, ** P <0.01
3.2 Ganoderma spore oil attenuation results in normal mice, H22 tumor-bearing mice 60Co radiotherapy, such as shown in Tables 3 and 4, compared with the control group, 60Co can significantly reduce the normal mice and tumor-bearing mouse peripheral blood WBC, bone marrow nucleated cells in the thymus and spleen coefficient (P <0.01). Compared with 60Co model group, Ganoderma spore oil in the high-dose group and the versicolor intracellular glycopeptide group could significantly inhibit the coefficient of normal and tumor-bearing mice spleen, thymus, WBC count and bone marrow nucleated cells decreased (P <0.01, P <0.05).
Table 3 ganoderma spore oil attenuated effect of normal mice 60Co radiotherapy (omitted)
Compared with the control group, P <0.05, P <0.01; compared with soybean oil 60 Co group, * P <0.05, ** P <0.01, n = 10
Table 4 Ganoderma spore oil of the H22 tumor 60Co radiotherapy attenuated (slightly)
Compared with the control group, P <0.05, P <0.01; compared with soybean oil 60 Co group, * P <0.05, ** P <0.01, n = 10
3.3 Ganoderma spore oil on H22 tumor reticuloendothelial cells the phagocytosis of results as shown in Table 5. Compared with soybean oil control group, the Ganoderma spores oil each dose group and the versicolor intracellular glycopeptide group could significantly increase the H22 tumor clearance index K phagocytic coefficient values ??(P
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